Year: 2022 | Month: February | Volume 12 | Issue 1
Molecular Detection of Mycoplasma gallisepticum in Broiler Chicken Farms of Satara and Pune Districts of Maharashtra
A.D. Vanjari
P.P. Mhase
A.K. Barate
M.S. Budhe
U.M. Tumlam
P.D. Pawar
D.M. Muglikar
DOI:10.30954/2277-940X.01.2022.5
Abstract:
Mycoplasma gallisepticum is one of most important pathogen economically affecting poultry worldwide. In the present study, filed samples from broiler flocks showing respiratory distress were analyzed by specific 16S rRNA PCR. The PCR products were sequenced and analyzed in silico by bioinformatics tool. At the flock level, 04 out 11 (36.36%) flocks were found positive for presence M. gallisepticum infection. Further, 7.57 % samples from Bhor and 6.06 % samples from Wai tested positive for presence of M. gallisepticum with PCR. In silico sequencing analysis revealed that field M. gallisepticum sequence was 100% identical with M. gallisepticum PB1/06/Ind sequence whereas it was 99.5% identical with f-99, ts-11 and 6/85 M. gallisepticum vaccine strains.
Highlights
- Mycoplasma gallisepticum is the most virulent and economically important pathogen causing chronic respiratory disease (CRD) in chickens.
- In silico analysis of nucleotide sequence of field Mycoplasma gallisepticum.
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